Lab Repotrt Essay - 1656 Words

Title: Comparing fish protein Introduction: Darwins Theory of Evolution is the commonly thought notion that all life is linked and has descended from a common ancestor. Darwins general theory assumes the development of life from nonlife and stresses a purely naturalistic descent with modification. That is, complex creatures evolve from more simplistic ancestors naturally over time. â€Å"Natural selection is a process in which individuals that have certain inherited traits tend to survive and reproduce at higher rates than other individuals because of those traits† (Campbell, 2014). How Darwin came about to these findings was by exploring the Galapagos Islands. It took years of research for him to come up with conclusions. He focused on†¦show more content†¦Our first step was that we boiled the samples, AM Std , Kaleidescope Std. for 5min. in water bath, then we loaded the samples into the wells following the guide given which was as follows: 1- 10 ml of laemmli buffer, 2- 10 ml of molecular weig ht, 3- 10 ml of salmon, 4- 10 ml of tilapia, 5- 10 ml of catfish, 6- 10 ml of shrimp, 7- 10 ml of actin and myosin, and 8- 10 ml of the laemmli buffer. To load each sample, we used a P-200 (yellow) micropipette tip to withdraw 10 ml of each protein sample from its tube and gently transferred it into the designated well. After loading all samples, on both of the gels we then placed the lid on the tank, and insert the leads into the power supply, matching red to red and black to black. We ran the gel for 45 minutes at a constant voltage of 115V. When the gels were finished running, we discarded the buffer from the inner chamber, we released the cams, and removed the gel cassettes from the assembly. We laid each gel cassette flat on the bench with the short plate facing